How were the two proteins used in this study produced?

How were the two proteins used in this study produced?

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(Questions are based on the attachments) One sentence or two sentence paragraph for each question

  1. What is the resolution of the Luo et al. (2012) RIG-I structure, and how does it compare with the resolution of the previously determined RIG-I structure used here as a search model?
  2. Looking at the structure of RIG-I reported here (4AY2), what is the distance between the N-terminal of the protein and the beta phosphate of the bound ADP  (Hint: Use the “Measurement” option under the Wizard menu tab in Pymol).
  3. How were the two proteins used in this study produced?
  4. How does the residue F853 interact with RNA?

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